Pilot Scale Production
have the capability to run gram level of protein purification. We
offer this service only for our preferred clients because of our limited
Cell cultivation can
be achieved in two 20L stirred tanks, shaking flasks(30L total), or fermentors(2L,
twin 3L, and 42L).
Cell harvest can be done
through hollow fiber ultra filtration at 30~120L/h
Cell disruption can be worked
out at 8000~10000ml/h at 15000psi
Liquid chromatography can
be run at 200~1600ml/min
Concentration can be carried
out at 500~2000ml/min
can be performed at 1000~2000ml of sample volume.
1000ml GST-Tag affinity column,
His-tag affinity column, 8000ml gel filtration column, and 8000ml
IEX column are also available. For those finest purification
projects, we have a Pharmacia Biopilot system
two P-6000 pumps, 100ml/min gradient, 2Mpa), and a Waters
Advance protein purification system (4 solvants, 45ml/min gradient,
750psi), a Waters prep LC3000 system (4
solvants, 80ml/min, 2500psi), and 3 ABI
BioCAD (BioCAD, BioCAD-RPM, BioCAD250,
6 solvant, 250ml/min, 3000psi).
AP-5 high pressure Glass columns (100-1200ml, 1000psi) are also
available to pack your tested resins in the pilot scale production.
4 regular FPLC systems (10ml/min,
4MPa), and a Bio-Rad biologic system (10ml/min,
1000psi), equipped with MonoQ, MonoS, reversed phase, and hydrophobic columns,
can be used to optimize the separation conditions. Electroelution
system from ABI (analytic) and Bio-Rad (preparative) are also